LIPE ELISA Kit (Bovine)

Cat# OKEH06635

Size : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Lipe ELISA Kit (Bovine) (OKEH06635) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Lipe has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Lipe is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Lipe captured in the well.
ELISA Kit Range0.156-10ng/ml
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Lipe Microplate96 Wells (12 x 8 Well strips)
Lipe Lyophilized Standard2 x 10 ng
100X Lipe HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.057 ng/mL
SpecificityNatural and recombinant Bovine Hormone-sensitive lipase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolLIPE
Gene Full Namelipase E, hormone sensitive type
Alias Symbolshormone-sensitive lipase;hsl;lipase, hormone-sensitive;monoacylglycerol lipase LIPE;REH;retinyl ester hydrolase.
NCBI Gene Id286879
Protein NameP16386
Description of TargetHormone-sensitive lipase
Uniprot IDhttps://www.uniprot.org/uniprot/P16386
Protein Accession #NP_001073689
Nucleotide Accession #NM_001080220