M9 Minimal salts 5x, Powder 52.5 g/L
Cat# 48505.01
Size : 525G
Marca : SERVA Electrophoresis
For cultivation of E. coli and plasmid amplification in molecular biology
30 g/l Na2HPO4
15 g/l KH2PO4
5 g/l NH4Cl
2.5 g/l NaCl
For making 1 L 5x concentrate dissolve 52.5 g in 1 L distilled water and sterilize by autoclaving. The 5x concentrate can be stored and diluted as needed to prepare 5 L of 1x M9 minimum salts.
References:
- Sambrook, J., et al., Molecular Cloning : A Laboratory Manual, 2nd ed., p. A.3, Cold Spring Harbor laboratory Press, Cold Spring Harbor, New York
HS: 38210000
Storage Temperature: +15 °C to +30 °C