Mouse Aspartate Aminotransferase (AST) Chemiluminescent Immunoassay (CLIA) Kit is a Sandwich Chemiluminescent Immunoassay (CLIA) Kit for use with Serum, plasma, tissue homogenates and other biological fluids.
Research Articles on Aspartate Aminotransferase, Cytoplasmic (GOT1)
| Introduction | Aspartate aminotransferase (ASAT, or aspartate transaminase, AST, gene symbol GOT1) is an enzyme that switches the amine group of aspartate with the ketone group of 2-oxoglutarate to form glutamate and oxaloacetate, and vice versa. This enzyme requires pyridoxal 5’-phosphate (a vitamin B6 derivative) to function, as the reaction uses this molecule to transfer the amine group. Serum GOT1 increases in response to a variety of afflictions, including liver injury, acute haemolytic anaemia, myocardial infarction, and severe burns. |
| Target | Aspartate Aminotransferase, Cytoplasmic (GOT1) |
| Reactivity | Mouse |
| Tested Applications | CLIA |
| Recommended dilutions | Optimal dilutions/concentrations should be determined by the end user. |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | The validity for this kit is at least 6 months. Up to 12 months validity can be provided on request. |
| Stability | The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout. |
| Test Range | 27.4 pg/ml - 20000 pg/ml |
| Sensitivity | < 10.4 ng/ml |
| Standard Form | Lyophilized |
| Detection Method | Chemiluminescent |
| Assay Type | Sandwich |
| Assay Data | Quantitative |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. |
| Availability | Shipped within 5-20 working days. |
| Note | This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
| Standard | 20000 pg/ml |