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Mouse Monoclonal Anti-Involucrin (Squamous Cell Terminal Differentiation Marker) [Clone IVRN/827]
Cat# NB-36-00395-P0
Size : 20ug
Marca : Neo Biotech
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Printable Datasheet
Involucrin (Squamous Cell Terminal Differentiation Marker) Antibody [IVRN/827]
Catalog Number Formulation Size Price
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Tonsil stained with Involucrin Monoclonal Antibody (IVRN/827)
Applications & Dilutions
Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Summary
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence
Species Reactivity
Human
Research Areas
Autophagy, Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cytoplasm
Gene Name
IVL
Positive Control
MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Immunogen
Purified involucrin from human keratinocytes
Alternate Names
Involucrin, IVL
Functions
- Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.
Key References
- Eckert, R.L. and Green, H. 1986. Structure and evolution of the human involucrin gene. Cell 46: 583-589.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2873896/
- https://pubmed.ncbi.nlm.nih.gov/16710414/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/9115270/
- https://pubmed.ncbi.nlm.nih.gov/1601889/
- https://pubmed.ncbi.nlm.nih.gov/2461365/
- https://pubmed.ncbi.nlm.nih.gov/9651377/
- https://pubmed.ncbi.nlm.nih.gov/15147942/
- https://pubmed.ncbi.nlm.nih.gov/21269460/
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic lossresulting from this product.
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