Product Description  

Mut Express II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on the ClonExpress homologous recombination technology for introducing single or double point mutations in plasmids. The kit integrates the Phanta Max Super-Fidelity DNA Polymerase amplification system and the ClonExpress rapid cloning system. With superior fidelity, Phanta Max significantly reduces the possibility of introducing novel mutations during amplification. Its excellent long fragment amplification ability is widely applicable to the amplification of any plasmid less than 20 kb. The ClonExpress rapid cloning system replaces the conventional annealing-cyclization reactions with the more efficient homologous recombination reaction. Site-directed mutagenesis using the Mut Express II Fast Mutagenesis Kit V2 allows a more flexible primer design and extremely low template input, thereby facilitating complete degradation of the initial methylated template. The highly optimized recombination reaction buffer and enhanced recombinase Exnase II significantly improve the efficiency of recombinant cyclization and tolerance to impurities. If the amplification product is highly specific, its DpnI-digested product can be used directly in the recombination reaction without DNA purification.

 Features  

• Low template usage: Robust and reliable exponential amplification method

• High-fidelity enzyme: Phanta Max Super-Fidelity DNA Polymerase (~128X Taq) minimizes unwanted secondary mutations

• Simple operation: Amplified product can be directly used in the recombination reaction after being digested with DpnI

• Widely applicable: Suitable for amplification of any site in the plasmid within 20 kb

• Software support: Provide online primer design software ‘‘CE Design’’

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤0°C.

▲Please avoid repeated freezing and thawing.