Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 
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Cat# GTX134510-25ul

Size : 25ul

Marca : GeneTex

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Telefono : +1 850 650 7790

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in ELISA (ELISA). GTX134510
Anti-beta Amyloid (1-42) antibody – Conformation Specific used in ELISA (ELISA). GTX134510
Anti-beta Amyloid (1-42) antibody – Conformation Specific used in IHC (Paraffin sections) (IHC-P). GTX134510
Anti-beta Amyloid (1-42) antibody – Conformation Specific used in IHC (Paraffin sections) (IHC-P). GTX134510
Anti-beta Amyloid (1-42) antibody – Conformation Specific used in IHC (Paraffin sections) (IHC-P). GTX134510
Anti-beta Amyloid (1-42) antibody – Conformation Specific used in Dot blot (Dot). GTX134510
Anti-beta Amyloid (1-42) antibody – Conformation Specific used in IHC (Paraffin sections) (IHC-P). GTX134510
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-P, Dot, ELISA, Neutralizing/Inhibition, Sandwich ELISA

Reactivity

 Human, Mouse
Package
100 μl,
25 μl
Datasheet File
View product citations for antibody GTX134510 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:1000
Dot Assay dependent
ELISA 1:1000-1:10000
Neutralizing/Inhibition Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635160, Detection: GTX134510
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Product Note

This antibody specifically recognizes beta amyloid aggregates but not peptide monomers.

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human Beta amyloid (1-42)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887299

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

amyloid beta precursor protein , AAA , ABETA , ABPP , AD1 , APPI , CTFgamma , CVAP , PN-II , PN2 , preA4

Cellular Localization

Cell membrane,Membrane,Cytoplasmic vesicle,Cytoplasm

Background

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Aug 2014]

Database

DATA IMAGES

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in ELISA (ELISA). GTX134510

GTX134510 ELISA Image

Sandwich ELISA detection of beta Amyloid (1-42)–peptide aggregate using GTX635160 as capture antibody at concentration of 10 μg/mL and GTX134510 as detection antibody at concentration of 0.45 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:2000 and used to detect the primary antibody.

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in ELISA (ELISA). GTX134510

GTX134510 ELISA Image

ELISA plate is coated with 100 μL of beta Amyloid (1-42) peptide aggregate at 10 μg/mL. The coated protein is detected with beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) at concentrations ranging from 45 ng/mL down to 1.67 ng/mL.

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in IHC (Paraffin sections) (IHC-P). GTX134510

GTX134510 IHC-P Image

Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the hippocampus of 3xTg-AD transgenic mouse brain (left), but not in age- and gender- matched normal mouse brain (right).
Sample: Paraffin-embedded 3xTg-AD transgenic mouse brain (left) and healthy mouse brain (right).
beta Amyloid (1-42) stained by beta Amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0
This image was provided courtesy of a customer review.

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in IHC (Paraffin sections) (IHC-P). GTX134510

GTX134510 IHC-P Image

Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the occipital lobe of human Alzheimer's disease brain by immunohistochemical analysis.
Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in IHC (Paraffin sections) (IHC-P). GTX134510

GTX134510 IHC-P Image

Beta amyloid (1-42) antibody (conformation specific) detects beta amyloid protein aggregates by immunohistochemical analysis in the occipital lobe of Alzheimer's disease brain (left), but not in normal human brain(right).
Sample: Paraffin-embedded Human Brain (Alzheimer's Disease + Normal) tissue slides (GTX49382).
beta amyloid protein aggregates stained by beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in Dot blot (Dot). GTX134510

GTX134510 Dot Image

Dot blot analysis of 200ng beta amyloid peptide (1-42) oligomers or monomers, using Beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:4000, 1:8000, and 1:16000.

Anti-beta Amyloid (1-42) antibody – Conformation Specific used in IHC (Paraffin sections) (IHC-P). GTX134510

GTX134510 IHC-P Image

Conformation-specific beta amyloid antibody detects beta amyloid protein aggregates in the postcentral gyrus of human Alzheimer's disease brain by immunohistochemical analysis.
Antibodies: beta amyloid (1-42) antibody – Conformation Specific (GTX134510) diluted at 1:500, and competitor's antibody diluted at 1:500.

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Application Reference

Tammaro A et al. iScience 2024; 27 (1) : 108681 HDAC1/2 inhibitor therapy improves multiple organ systems in aged mice.
Yang Y et al. Front Aging Neurosci 2023; 15 : 1126273 A single dose of lipopolysaccharide elicits autofluorescence in the mouse brain.
Reactivity : Mouse
Amano A et al. J Nanobiotechnology 2023; 21 (1) : 36 Peripheral administration of nanomicelle-encapsulated anti-Aβ oligomer fragment antibody reduces various toxic Aβ species in the brain.
Application : Neutralizing/Inhibition
Wang H et al. CNS Neurosci Ther 2021; (Epub) Endothelial genetic deletion of CD147 induces changes in the dual function of the blood-brain barrier and is implicated in Alzheimer's disease.
Application : IHC-P
Reactivity : Mouse
Dinkel F et al. Front Aging Neurosci 2020; 12 : 220 Decreased Deposition of Beta-Amyloid 1-38 and Increased Deposition of Beta-Amyloid 1-42 in Brain Tissue of Presenilin-1 E280A Familial Alzheimer's Disease Patients.
Application : IHC-P
Reactivity : Human

REVIEW

beta Amyloid (1-42) antibody – Conformation Specific Cat. No. GTX134510

Rating
( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-beta Amyloid (1-42) antibody – Conformation Specific used in IHC (Paraffin sections) (IHC-P). GTX134510
Date : Anonymous submitted on 10-Sep-2018
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Hippocampus
Primary Antibody : 1:500, 4°C, 16Hr
Application Reference
Tammaro A et al. iScience 2024; 27 (1):108681 HDAC1/2 inhibitor therapy improves multiple organ systems in aged mice.
Yang Y et al. Front Aging Neurosci 2023; 15:1126273 A single dose of lipopolysaccharide elicits autofluorescence in the mouse brain.
Reactivity : Mouse
Amano A et al. J Nanobiotechnology 2023; 21 (1):36 Peripheral administration of nanomicelle-encapsulated anti-Aβ oligomer fragment antibody reduces various toxic Aβ species in the brain.
Application : Neutralizing/Inhibition
Wang H et al. CNS Neurosci Ther 2021; (Epub) Endothelial genetic deletion of CD147 induces changes in the dual function of the blood-brain barrier and is implicated in Alzheimer's disease.
Application : IHC-P
Reactivity : Mouse
Dinkel F et al. Front Aging Neurosci 2020; 12:220 Decreased Deposition of Beta-Amyloid 1-38 and Increased Deposition of Beta-Amyloid 1-42 in Brain Tissue of Presenilin-1 E280A Familial Alzheimer's Disease Patients.
Application : IHC-P
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

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