pNPP Substrate
Cat# 4400L
Size : 500ML
Marca : Kementec
Contatta il distributore locale :
Telefono : +1 850 650 7790
![Graph illustrating the long term stability of pNPP substrates](https://kementec.com/media/1436/pnpp.png?anchor=center&mode=crop&width=450&height=212&rnd=132228763130000000")
![Graph illustrating the Signal-to-Noise Ratio of pNPP substrates](https://kementec.com/media/1437/pnpp-2.png?anchor=center&mode=crop&width=450&height=212&rnd=132228763580000000")
pNPP (p-nitrophenylphosphate) is a chromogenic substrate for Alkaline Phosphatase (AP). In the presence of AP, pNPP is hydrolyzed rapidly to p-nitrophenol and inorganic phosphate. Liberated p-nitrophenol is seen as a clear yellow color that is measured at 405/620 nm. pNPP liquid ready-to-use substrate is highly active compared to pNPP ONE (cat. No. 4401). The substrate gives superior results due to its sensitivity, allowing very low detection limits.
The product is available in bulk quantities and as customized solutions.
Specifications
- Available sizes: 100 mL, 500 mL, 1 L, bulk
- Format: Ready-to-use (RTU)
- Storage: 2-8⁰C in the dark
- Stability: 2 years
- Development time: 5-30 minutes
- Stop Solution: 0,1 M NaOH
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