POU5F1 (POU Domain, Class 5, Transcription Factor 1, POU Domain Protein 2, pou5f1, gp-9, pou-2, pou2) (APC)

Cat# 302658-APC-100ul

Size : 100ul

Marca : US Biological

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302658-APC POU5F1 (POU Domain, Class 5, Transcription Factor 1, POU Domain Protein 2, pou5f1, gp-9, pou-2, pou2) (APC) discontinued

Clone Type
Polyclonal
Host
rabbit
Source
Zebrafish
Swiss Prot
Q90270
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Ze
Gene #
pou5f1
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation.||Applications: |Suitable for use in Western Blot. Other applications not tested.||Recommended Dilution:|Western Blot: 1:2000|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: Zebrafish|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography.|Immunogen: KLH conjugated synthetic peptide corresponding to aa141-177 at the Central region of Zebrafish pou5f1.|Specificity: Recognizes zebrafish pou5f1. Species Crossreactivity: zebrafish||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
KLH conjugated synthetic peptide corresponding to aa141-177 at the Central region of Zebrafish pou5f1.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes zebrafish pou5f1. Species Crossreactivity: zebrafish
References
Takeda H.,et al.Genes Dev. 8:45-59(1994).|Hauptmann G.,et al.Mech. Dev. 51:127-138(1995).|