WST-1 [150849-52-8]
Cat# HY-136976-1ml
Size : 10mM/1mL
Marca : MedChemExpress
WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm.
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WST-1 Chemical Structure
CAS No. : 150849-52-8
This product is a controlled substance and not for sale in your territory.
Based on 2 publication(s) in Google Scholar
Description |
WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm[1]. |
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In Vitro |
Preparation of WST-1 working solution MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Masse moléculaire |
651.34 |
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Formule |
C19H11IN5NaO8S2 |
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CAS No. |
150849-52-8 |
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Appearance |
Solid |
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Color |
Light yellow to brown |
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SMILES |
O=[S]([O-])(C1=C(C=CC([S](=O)(O[Na])=O)=C1)C2=NN(C(C=C3)=CC=C3I)[N+](C(C=C4)=CC=C4[N+]([O-])=O)=N2)=O |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
4°C, sealed storage, away from moisture and light *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light) |
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Solvant et solubilité |
In Vitro:
H2O : 125 mg/mL (191.91 mM; Need ultrasonic) DMSO : 25 mg/mL (38.38 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
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Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Pureté et documentation |
Purity: ≥98.0% |
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Références |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO / H2O | 1 mM | 1.5353 mL | 7.6765 mL | 15.3530 mL | 38.3824 mL |
5 mM | 0.3071 mL | 1.5353 mL | 3.0706 mL | 7.6765 mL | |
10 mM | 0.1535 mL | 0.7676 mL | 1.5353 mL | 3.8382 mL | |
15 mM | 0.1024 mL | 0.5118 mL | 1.0235 mL | 2.5588 mL | |
20 mM | 0.0768 mL | 0.3838 mL | 0.7676 mL | 1.9191 mL | |
25 mM | 0.0614 mL | 0.3071 mL | 0.6141 mL | 1.5353 mL | |
30 mM | 0.0512 mL | 0.2559 mL | 0.5118 mL | 1.2794 mL | |
H2O | 40 mM | 0.0384 mL | 0.1919 mL | 0.3838 mL | 0.9596 mL |
50 mM | 0.0307 mL | 0.1535 mL | 0.3071 mL | 0.7676 mL | |
60 mM | 0.0256 mL | 0.1279 mL | 0.2559 mL | 0.6397 mL | |
80 mM | 0.0192 mL | 0.0960 mL | 0.1919 mL | 0.4798 mL | |
100 mM | 0.0154 mL | 0.0768 mL | 0.1535 mL | 0.3838 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
WST-1 Related Classifications
- Cancer
- Cancer Immunotherapy Cancer Metabolism and Metastasis
- Others
- Fluorescent Dye
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
WST-1150849-52-8WST1WST 1Fluorescent DyeformazancellviabilityNADPHmitochondrialdehydrogenaseInhibitorinhibitorinhibit