Canvax Biotech
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Canvax is a leading manufacturer and supplier of the most innovative solutions, kits and R&D Reagents inside Molecular Biology fields. Since its foundation in 2001, Canvax offers reliable, cost-effective and easy-to-use innovative tools for research. Based in Córdoba (Spain), since its beginning it has focused on R&D of multiplex high throughput platforms (HTS) for Drug discovery and Diagnostic applied biosensors. Over a decade later, Canvax is a worldwide leading expert in GPCR expression in heterologous cells, with important patents and exclusive know-how. Canvax prides itself to be the first company to get an unprecedented milestone that could revolutionize the Diagnostics sector: Canvax established stable high-level expression of odour GPCRs into heterologous cell lines in 2014. This key milestone, that could be applied in diagnostic, perfume and cosmetic industry, are being employed to obtain a molecular nose, a sensitive and non-invasive diagnostic molecular device for early detection of cancer, as the nose of trained dogs do. Thanks to this project, Canvax was awarded, in 2013, with the largest Public & Private Contract for Pre competitive Technology Development in Spanish history. Website : www.lifescience.canvaxbiotech.com | ||
qPCR mix
MasterMix is a ready-to-use reagent in which all the components are already mixed in an optimized way. Additional stabilizers are added to enable long-term storage.
The concentration of components is perfectly adapted to most of the assays.
Taq Polymerase
A HotStart Taq polymerase is inactive at low temperatures (room temperature). Heating at 95 °C activates the enzyme, and the amplification can begin once the primers are annealed. The enzyme is not active until the entire DNA is denatured.
dNTPs / dUTPs
Some kits contain a blend of dNTPs and dUTPs, other ones contain only dNTPs.
UNG
The Uracil-N-Glycosylase is an enzyme that hydrolyses all single-stranded and double-stranded DNA containing dUTPs.
ROX
Some thermocyclers require MasterMix containing ROX dye for normalization. This is the case for the ABI and Eppendorf machines, and optional on the Stratagene machines.
Fluorescein
For some thermocyclers, the normalization method for SYBR® Green assay uses Fluorescein to create a “virtual background”.
MgCl2
MgCl2 is necessary for the Reverse Transcriptase and the Taq activity. MgCl2 concentration in MasterMixes is optimized according to the amount of Taq and also the buffer composition.
Inert colored dye
Some buffers also include an inert colored dye, to enable visualization of the buffer when loading in the wells. This colored dye has no effect on the sensitivity of the assay and is a convenient working tool.
