Canvax Biotech
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Canvax is a leading manufacturer and supplier of the most innovative solutions, kits and R&D Reagents inside Molecular Biology fields. Since its foundation in 2001, Canvax offers reliable, cost-effective and easy-to-use innovative tools for research. Based in Córdoba (Spain), since its beginning it has focused on R&D of multiplex high throughput platforms (HTS) for Drug discovery and Diagnostic applied biosensors. Over a decade later, Canvax is a worldwide leading expert in GPCR expression in heterologous cells, with important patents and exclusive know-how. Canvax prides itself to be the first company to get an unprecedented milestone that could revolutionize the Diagnostics sector: Canvax established stable high-level expression of odour GPCRs into heterologous cell lines in 2014. This key milestone, that could be applied in diagnostic, perfume and cosmetic industry, are being employed to obtain a molecular nose, a sensitive and non-invasive diagnostic molecular device for early detection of cancer, as the nose of trained dogs do. Thanks to this project, Canvax was awarded, in 2013, with the largest Public & Private Contract for Pre competitive Technology Development in Spanish history. Website : www.lifescience.canvaxbiotech.com | ||
Reverse transcriptase PCR - RT-PCR
RT-PCR is a technique which makes it possible to make a PCR (polymerase chain reaction) from an RNA sample. RNA is first retrotranscribed by an enzyme called reverse transcriptase, which allows the synthesis of the complementary DNA (cDNA). The latter is then used to carry out a PCR. Reverse transcriptase or reverse transcriptase (RT) is an enzyme used by retroviruses and retrotransposons that transcribe the genetic information of viruses or retrotransposons from RNA to DNA, which can integrate into the genome of the host.
RT-PCR has been developed to use RNAs as a PCR amplification matrix. It is certainly the most sensitive method for detecting (and possibly quantifying) messenger RNAs at the level of an organ, tissue or cell. For this purpose, it is often carried out in situ (PCR in situ), ie on fixed biological material. It is also used for the construction of cDNA libraries, sorting of mRNA (Differencial Display RT-PCR) as well as the construction of DNA probes.
