Reagenti e strumenti per immunologia, biologia cellulare e biologia molecolare
 

TransGen Biotech

TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. 

Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies. 
 
 
> TransGen Biotech > Prodotti per la ricerca > Strumenti di biologia molecolare > Sequenziamento di nuova generazione (NGS) > NGS Library quantification products > Library quantifcation master mix for Illumina®
Library quantifcation master mix for Illumina®

Library quantifcation master mix for Illumina®

Accurate quantitation of the number of molecules in a library is the most critical step in the preparation steps for next-generation sequencing (NGS) to obtain high-quality reading data. Indeed, in order to obtain reliable and quality reading data, it is necessary to use a sufficient quantity of DNA, and in particular for Illumina® sequencing. The use of insufficient DNA will result in low sequencing efficiency and DNA overabundance will increase cluster density and result in poor quality data.

Quantitative PCR is considered to be the most accurate and efficient method of quantification of NGS library.

The library quantitation kits for Illumina® sequencing contain all the components bellow:
  • DNA polymerase
  • dNTPs
  • MgCl2
  • Primers to amplify only the molecules that contain the two sequences of adapters
  • SYBR green or EvaGreen
  • A ROX solution to add depending on the device used. If the ROX solution is separate from the mix, it is a universal kit that can be used with all instruments. If ROX is contain in the master mix, it is necessary to use the appropriate kits with your device.

UniversalAll instruments
Without ROXBio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4;
Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s;
Illumina Eco qPCR;
Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000;
Roche Applied Science LightCyclerTM 480;
Thermo Scientific PikoReal Cycler.
Low ROXApplied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000,
MX3005P, MX3000P
High ROXApplied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast;
StepOne, StepOnePlus


Risultati della ricerca : 2 prodotto(i) trovato(i)

Limita la ricerca :

RUOCE / IVD
  • kit 2
  • PCR 2
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