TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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NGS Transcriptome sequencing - Library preparation
The term RNA-Seq or RNA-Seq refers to a next-generation sequencing approach (NGS) that provides a snapshot of the entire transcriptome or messenger RNA (mRNA) profile at a given point in time.
Whole transcriptome analysis aims at capturing coding and non-coding RNA and quantifying the heterogeneity of gene expression in cells, tissues, organs and even the entire body.
The sequencing of the whole transcriptome therefore provides the most complete view possible. It is the method that allows the sequencing of all transcripts of coding and non-coding RNA. In other words, the messenger RNAs that code for proteins but also all other micro RNAs, small interfering RNAs, extracellular RNAs.
The other advantage of whole genome sequencing is that the extraction and purification of the total RNA is easier and cheaper than messenger RNA for example. However, it is necessary to remove the ribosomal RNA that is present in too large quantities and that could interfere with other types of RNA. This is called ribosomal RNA depletion.
