Next-generation reverse transcriptases (RTs) represent a significant advancement in molecular biology, particularly in the synthesis of complementary DNA (cDNA) from RNA templates. These enzymes have been engineered to overcome limitations of traditional RTs, enhancing their efficiency, fidelity, and applicability in various research contexts.

Next-generation RTs are pivotal in various applications:

• RNA Sequencing (RNA-Seq): They enable the generation of high-quality sequencing libraries necessary for comprehensive transcriptomic studies, allowing researchers to explore gene expression patterns more effectively than with conventional methods
• Synthetic Biology: Engineered RTs facilitate the synthesis and manipulation of synthetic genetic polymers (xeno nucleic acids or XNAs), expanding the toolkit available for synthetic genetics and molecular engineering
• Genome Editing: Novel applications include coupling engineered RTs with CRISPR/Cas9 systems for precise genome editing, showcasing their versatility beyond traditional uses