SDS precast gels with a gradient of 4-20% are vital tools in protein electrophoresis, specifically designed to facilitate the separation of proteins across a broad molecular weight range. These gels feature a gradual increase in polyacrylamide concentration from 4% at the top to 20% at the bottom, allowing for effective resolution of proteins ranging from small peptides to larger protein complexes.

Key Features

1. Gradient Structure: The unique gradient enables the separation of a diverse array of protein sizes within a single gel run. Larger proteins migrate more easily through the larger pores at the top, while smaller proteins are better resolved as they encounter progressively smaller pores toward the bottom.
2. Precast Convenience: Being precast eliminates the variability and time associated with hand-casting gels, ensuring consistent results and streamlined workflows in laboratory settings. This ready-to-use format is particularly beneficial for high-throughput applications.
3. Enhanced Performance: These gels are optimized for high-resolution separation, providing sharp and distinct bands that are essential for accurate analysis in subsequent techniques like Western blotting. They also offer faster run times compared to traditional single-percentage gels.
4. Versatile Applications: SDS precast gels with a 4-20% gradient are suitable for various applications, including SDS-PAGE for denaturing protein analysis and Western blotting for protein detection and quantification.