Classical DNA polymérases for difficult templates

Classical DNA polymérases for difficult templates

PCR is a well known and effective tool for amplification of DNA sequences of interest. When DNA sequences with a high GC content are amplified, the formation of PCR products can often be compromised by inadequate strand separation and by the propensity for the formation of complex secondary structures. The inability of the DNA polymerase to function through regions of strong secondary structure, such as "hairpins", often leads to the formation of truncated PCR products. In addition, poorly defined amplification products are prevalent when the primers are GC-rich. Despite the inherent challenges, the detection of GC-rich sequences is becoming increasingly important for the molecular diagnosis of hereditary diseases.
Successful amplification of difficult samples (eg, sequences containing GC-rich regions) often requires the addition of co-solvents (eg, dimethylsulfoxide (DMSO)) to lower the separation temperature of the DNA strand. However, this method has the disadvantage of inhibiting enzymatic activity and slowing the rate of extension - which is a problem if only a small amount of DNA is available.

We propose DNA polymerases with robust performance in a wide range of models rich in GC and AT. These DNA polymerases have improved tolerance to common PCR inhibitors for crude samples and / or DNA extractions.


Name Neo-Taq Robust DNA Polymerase Taq DNA polymerase + Robust buffer
Product code NB-33-0001 02-012 and 02-002
Type Specially blended enzyme mix containing DNA polymerase (a 5'-exo-minus, N-terminal deletion of Taq DNA polymerase) and a small amount of a proofreading DNA polymerase Taq DNA polymerase with optimized buffer for difficult samples
Speed 3 kb/min 4 kb/min
Amplicon size < 10 kb < 14 kb
3'-5' exonuclease activity Yes No



Risultati della ricerca : 47 prodotto(i) trovato(i)

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RUOCE / IVD
  • Unconjugated 4
  • polymerase 4
  • Biochemicals 33
  • Inhibitor/Antagonist/Agonist 6
  • Equipment 4
  • Polymerase 4
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Cat#
Descrizione
Cond.
Priced
NB-12-4010B
 1unit 
NB-12-4010A
 1unit 
orb1695425-25mg
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T34380-25mg
 25mg 
orb2279353-5mg
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orb2279423-5mg
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T125802-5mg
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T125170-1mg
 1mg 
T125170-5mg
 5mg 
orb2279423-1mg
 1mg 
orb2279353-1mg
 1mg 
T125802-1mg
 1mg 
orb2279330-1mg
 1mg 
T126133-1mg
 1mg 
orb2279330-5mg
 5mg 
T126133-5mg
 5mg 
orb1987013-100mg
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T26111-500mg
 500mg