TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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Cloning tools
DNA cloning or molecular cloning is the process of making multiple identical copies of a particular piece of DNA. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for the replication of the recombinant DNA. Molecular cloning methods are at the heart of many contemporary fields of modern biology and medicine. In a typical DNA cloning procedure, the gene or other DNA fragment of interest is first inserted into a circular piece of DNA called a plasmid. The insertion is performed using enzymes that "cut and paste" the DNA, and it produces a recombinant DNA molecule, or DNA assembled from fragments from multiple sources.
Virtually any DNA sequence can be cloned and amplified, but certain factors can limit the success of the process. Examples of DNA sequences that are difficult to clone are reverse repeats, origins of replication, centromeres and telomeres. Another characteristic that limits the chances of success is the large size of the DNA sequence. Insertions larger than 10 kbp have very limited success, but bacteriophages such as the bacteriophage λ can be modified to successfully insert a sequence up to 40 kbp.
