TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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Transfection reagents
Transfection is the process of introducing exogenous genetic material in the form of DNA or RNA into eukaryotic cells. The entry of this genetic material into the cell is allowed by the transient opening of pores in the membranes, it is then possible to introduce a plasmid, a small interfering RNA or other. Cells manipulated to accept a foreign nucleic acid are called competent cells. Transfection is not to be confused with transduction, which uses a different route of entry into the cell (through the use of a viral vector).
There are different methods of transfection, which are different chemical and/or physical treatments that will make the plasma membrane more permeable. The main methods used are :
- Precipitation with calcium phosphate: the DNA will adhere to the membrane and enter the cell.
- Lipofection, which will allow the DNA to be associated with cationic lipids, will create liposomes that will enter the cell by endocytosis.
- Electroporation, which uses an electrical pulse to depolarize the membrane and open the pores.
