TransGen Biotech
 |
||
TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
| ||
Reagents for PCR
Polymerase chain reaction (PCR) reagents are critical components in the amplification of specific regions of DNA. These reagents play a vital role in ensuring the accuracy, sensitivity, and specificity of PCR, which is a widely used technique in molecular biology research.
One of the most important PCR reagents is the DNA polymerase enzyme, which is responsible for catalyzing the synthesis of new DNA strands during PCR. There are various types of DNA polymerases available for PCR, each with its own unique properties and advantages. For example, Taq polymerase is a commonly used DNA polymerase that is highly thermostable and can withstand the high temperatures required for PCR denaturation.
In addition to DNA polymerase, other PCR reagents include deoxynucleotide triphosphates (dNTPs), which serve as the building blocks for the new DNA strands; PCR buffers, which provide the optimal chemical environment for DNA polymerase activity; and various PCR additives, such as betaine and DMSO, which can enhance PCR performance in certain applications.
