TransGen Biotech
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TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research. Currently, our products cover: plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
Website : www.transgenbiotech.com
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Kits for the preparation of DNA libraries for NGS
Library preparation for Illumina is composed of 4 main steps, the progress of which can vary according to the methods used:
1. DNA fragmentation
DNA is fragmented either enzymatically or by sonication to create smaller strands
2. End repair
Because DNA fragmentation does not result in homogeneous, blunt-ended fragments, end repair is needed to ensure that each molecule is free of overhangs, and contains 5' phosphate and 3' hydroxyl groups
3. Adapter ligation
Adaptors (short, double-stranded pieces of synthetic DNA) are then ligated to these fragments with the help of DNA ligase. The adaptors enable the sequence to become bound to a complementary counterpart. These adapters contain sequence motifs that are required for subsequent steps (clonal amplification and the actual sequencing).
4. Library amplification
Library amplification is required so that the received signal from the sequencer is strong enough to be detected accurately. The Illumina technology uses the "Bridge PCR" method.
