Molecular Dimensions
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Molecular Dimensions è un fornitore leader a livello mondiale di moderni schermi, reagenti, altri materiali di consumo e strumentazione per la determinazione della struttura delle proteine mediante cristallografia a raggi X.
Molecular Dimensions offre la gamma più innovativa di prodotti derivanti dagli ultimi sviluppi della ricerca in biologia strutturale. L'azienda ha attualmente oltre 20 progetti in varie fasi di sviluppo, da nuovi schermi e reagenti per la crescita dei cristalli, a nuovi articoli in plastica per il laboratorio, fino a una gamma di strumentazioni. Sito web: www.moleculardimensions.com
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Reducing agent - DTT
Dithiothreitol (DTT) is a potent reducing agent commonly used to break down protein disulfide bonds and stabilize enzymes and other proteins. It has a redox potential of -0.33 V at pH 7, making it an unusually strong reducing agent. DTT participates in disulfide exchange reactions and is used at concentrations of 1-10 mM for protein disulfide bond reduction. It is also capable of crossing biological membranes. DTT is used to prevent the formation of mixed-disulfide species and is frequently employed to reduce the disulfide bonds of proteins and prevent the formation of intramolecular and intermolecular disulfide bonds between cysteine residues of proteins. Additionally, DTT can be used as an oxidizing agent, with the principal advantage that effectively no mixed-disulfide species are populated, in contrast to other agents such as glutathione