SERVA Electrophoresis
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50 years of experience in the supply of quality reagents at SERVA provides expertise in manufacture, development and distribution of products worldwide. This know-how is the platform for outstanding customer service - all you need to succeed. Our mission is to bring innovative products to the life sciences market, meeting our customers needs. Technical competence and total quality management are the basis for continuous improvement. We pursue the highest standards in product quality, workplace safety and responsibility for the environment we live in. We dedicate our capacity and integrity to guarantee consistent product performance and continuity of supply. We are certified for ISO 9001:2000.
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Nucleic acids extraction
Nucleic acid extraction methods are essential to molecular biology and are commonly used in many medical and biological science applications.
Nucleic acid extraction can be divided into 3 steps, which can be optimized according to the type of sample and the downstream applications for which the nucleic acids will be used. These steps are as follows:
1) Rupture of tissue and cell structures
2) Removal of proteins, lipids and other contaminants from nucleic acids
3) Transfer the nucleic acids to water or a buffer solution that will preserve them without interfering with subsequent work.
Nucleic acid extraction methods can be classified into two different types :
- Solution methods (such as the phenol-chloroform method)
- Methods based on the solid phase (such as magnetic beads or centrifugation columns).
Solid-phase extraction methods work by causing nucleic acids to bind to solid supports, such as magnetic beads coated with silica or other materials. The beads (or other supports) are then washed with alcohol to remove contaminants. The support is then washed with a liquid that makes the nucleic acids soluble again, which releases the DNA from the support in a process known as elution. A simple method is to add a solution containing a chaotrope to the crude nucleic acid extract. Chaotropic agents are molecules that disrupt the hydrogen bonding network between water molecules. In the presence of chaotropes, nucleic acids are previously much less soluble and bind to glass, silica-covered magnetic beads and other solid supports - allowing them to be easily separated from contaminants. Solid-phase extraction methods may also depend on selective binding of DNA and RNA to ion exchange resins or other chemicals.