Biorbyt
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Products for advancing your discoveries From antibodies to proteins, biochemicals to ELISA kits, Biorbyt's high quality research products are available for you to enable world class research and contribution to a deeper understanding of life sciences.
A comprehensive catalog of life science research products covering the areas of Neuroscience, Signal transduction, Cell Biology, Cancer Metabolism, Cardiovascular and others
Product range :
Choose from over 100,000 primary antibodies and over 2,000 secondary antibodies. Monoclonals and polyclonals tested in a number of applications - WB, ELISA, IHC, FACS and many more.
Choose from over 7,000 proteins and over 400 active proteins. All of our proteins have been validated for use in ELISAs, WB and IHC-P
We have over 10,000 biochemicals for use in your research.
We have over 2,000 ELISA kits covering many research areas . Each kit comes with a fully detailed protocol and examples of expected results.
Website : https://www.biorbyt.com/
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![Reducing agent - TCEP](https://www.clinisciences.com/it/upload/thumbs/chimie-bandeau-q8n8tykn-abqpBs4c-464f93.jpg)
Reducing agent - TCEP
Tris(2-carboxyethyl)phosphine (TCEP) is a powerful reducing agent commonly used in biological and biochemical research. It is particularly effective in breaking disulfide bonds in proteins and maintaining a reducing environment. Here is an overview of its properties and applications:
Chemical Properties:
TCEP is a water-soluble compound with the chemical formula C9H15O6PS3. It contains a phosphine group, which is a strong reducing agent. The phosphine group is capable of donating electrons to break disulfide bonds in proteins, leading to the reduction of protein structures.
Biological and Biochemical Applications:
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Protein Biochemistry: TCEP is widely used in protein biochemistry to reduce disulfide bonds in proteins. This is essential for studies involving protein refolding, denaturation, or modification. TCEP is often preferred over other reducing agents like dithiothreitol (DTT) or 2-mercaptoethanol because it does not contain thiol groups and, therefore, does not contribute to S-methylation or other side reactions.
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Cell Culture: In cell culture, TCEP is used to maintain a reducing environment in media, preventing the formation of disulfide bonds in proteins. It can be added to cell culture media to protect cells from oxidative stress.
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Nucleic Acid Studies: TCEP is used in nucleic acid studies to reduce disulfide bonds in RNA and DNA extraction procedures. This helps prevent the oxidation of nucleic acids and maintains their integrity during isolation.
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Mass Spectrometry: TCEP is employed in mass spectrometry experiments, particularly in the analysis of proteins. Its use allows for the reduction of disulfide bonds before analysis, providing more accurate information about protein structure.
Safety Considerations:
TCEP is generally considered safe to handle, but, like any chemical reagent, precautions should be taken. It is essential to follow safety guidelines, including wearing appropriate personal protective equipment and working in a well-ventilated area.
Risultati della ricerca : 11 prodotto(i) trovato(i)
Limita la ricerca :
- Biochemicals 11