Biorbyt
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Products for advancing your discoveries From antibodies to proteins, biochemicals to ELISA kits, Biorbyt's high quality research products are available for you to enable world class research and contribution to a deeper understanding of life sciences.
A comprehensive catalog of life science research products covering the areas of Neuroscience, Signal transduction, Cell Biology, Cancer Metabolism, Cardiovascular and others
Product range :
Choose from over 100,000 primary antibodies and over 2,000 secondary antibodies. Monoclonals and polyclonals tested in a number of applications - WB, ELISA, IHC, FACS and many more.
Choose from over 7,000 proteins and over 400 active proteins. All of our proteins have been validated for use in ELISAs, WB and IHC-P
We have over 10,000 biochemicals for use in your research.
We have over 2,000 ELISA kits covering many research areas . Each kit comes with a fully detailed protocol and examples of expected results.
Website : https://www.biorbyt.com/
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![Sequencing of total RNA by NGS - Library preparation](https://www.clinisciences.com/it/upload/thumbs/ngs-4zz6kd-e5a1f4.jpg)
Sequencing of total RNA by NGS - Library preparation
The term RNA-Seq or RNA-Seq refers to a next-generation sequencing approach (NGS) that provides a snapshot of the entire transcriptome or messenger RNA (mRNA) profile at a given point in time.
Whole transcriptome analysis aims at capturing coding and non-coding RNA and quantifying the heterogeneity of gene expression in cells, tissues, organs and even the entire body.
The sequencing of the whole transcriptome therefore provides the most complete view possible. It is the method that allows the sequencing of all transcripts of coding and non-coding RNA. In other words, the messenger RNAs that code for proteins but also all other micro RNAs, small interfering RNAs, extracellular RNAs.
The other advantage of whole genome sequencing is that the extraction and purification of the total RNA is easier and cheaper than messenger RNA for example. However, it is necessary to remove the ribosomal RNA that is present in too large quantities and that could interfere with other types of RNA. This is called ribosomal RNA depletion.