K1034-5 | 2X Taq PCR Master Mix (with dye) Clinisciences

Product Citation

1. Dan Xie, et al. "NEIL1 drives the initiation of colorectal cancer through transcriptional regulation of COL17A1." Cell Rep. 2024 Jan 23;43(1):113654. PMID: 38175757
2. Jing-Yi Peng, Xuqing Liu, et al. "Early pheromone perception remodels neurodevelopment and accelerates neurodegeneration in adult C. elegans." Cell Rep. 2023 Jun 27;42(6):112598. PMID: 37289584

Description

Our product 2X Taq PCR Master Mix is a ready-to-use 2X premix solution containing Taq DNA Polymerase, dNTPs, an already optimized buffer system and dye. Taq DNA polymerase synthesizes DNA under appropriate conditions from single-stranded templates in the presence of the gene-specific primers and dNTPs. It’s a recombinant product extracted from E.coli with thermu aquaticus DNA polymerase gene expressed.

Taq DNA Polymerase possesses the capability of 5'→3' DNA polymerase and weak 5'→3' exonuclease, yet, no potential of 3'→5' exonuclease which means there will appear an dA overhang at the 3’ end. Using this characteristic, it can be used in TA cloning. In PCR reaction, elongation rate of Taq DNA Polymerase is about 1-2 kb/min depending on the complexity of the gene. For most templates, using 1kb/min.

The reaction system is ready to use by adding primers, templates and ddH₂O, simplifying the experimental steps, reducing personal error and improving the repeatability of the results.

This Master Mix contains dye which means it can be directly electrophoresed after the amplification without the need to add a loading buffer. If needed to subject the PCR product to agarose gel electrophoresis, our product SYBR Safe DNA Gel Stain (Cat: A8743) is available.