6-FAM SE [92557-81-8]
Cat# HY-15939-10mg
Size : 10mg
Marca : MedChemExpress
6-FAM SE is another isomer of carboxyfluorescein.
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6-FAM SE Chemical Structure
CAS No. : 92557-81-8
This product is a controlled substance and not for sale in your territory.
Description |
6-FAM SE is another isomer of carboxyfluorescein. 6-FAM, SE is mainly used in sequencing of nucleic acids and labeling nucleotides. |
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Masse moléculaire |
473.39 |
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Formule |
C25H15NO9 |
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CAS No. |
92557-81-8 |
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Appearance |
Solid |
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Color |
Yellow to orange |
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Emission (Em) |
515 |
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Excitation (Ex) |
488 |
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SMILES |
O=C(C1=CC(C2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)O5)=C(C=C1)C5=O)ON6C(CCC6=O)=O |
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Livraison | Room temperature in continental US; may vary elsewhere. |
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Stockage |
-20°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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Solvant et solubilité |
In Vitro:
DMSO : 50 mg/mL (105.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Pureté et documentation |
Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 2.1124 mL | 10.5621 mL | 21.1242 mL | 52.8106 mL |
5 mM | 0.4225 mL | 2.1124 mL | 4.2248 mL | 10.5621 mL | |
10 mM | 0.2112 mL | 1.0562 mL | 2.1124 mL | 5.2811 mL | |
15 mM | 0.1408 mL | 0.7041 mL | 1.4083 mL | 3.5207 mL | |
20 mM | 0.1056 mL | 0.5281 mL | 1.0562 mL | 2.6405 mL | |
25 mM | 0.0845 mL | 0.4225 mL | 0.8450 mL | 2.1124 mL | |
30 mM | 0.0704 mL | 0.3521 mL | 0.7041 mL | 1.7604 mL | |
40 mM | 0.0528 mL | 0.2641 mL | 0.5281 mL | 1.3203 mL | |
50 mM | 0.0422 mL | 0.2112 mL | 0.4225 mL | 1.0562 mL | |
60 mM | 0.0352 mL | 0.1760 mL | 0.3521 mL | 0.8802 mL | |
80 mM | 0.0264 mL | 0.1320 mL | 0.2641 mL | 0.6601 mL | |
100 mM | 0.0211 mL | 0.1056 mL | 0.2112 mL | 0.5281 mL |
6-FAM SE Related Classifications
- Others
- Fluorescent Dye
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
6-FAM SE92557-81-8Fluorescent DyeInhibitorinhibitorinhibit