Aconitase 1 (ACO1) (NM_002197) Human 3' UTR Clone
CAT#: SC209391
3' UTR clone of aconitase 1 soluble (ACO1) for miRNA target validation
Product Images
Specifications
| Product Data | |
| Vector | pMirTarget |
| Species | Human |
| Transfection Reporter | RFP |
| Assay Reporter | Luciferase |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Symbol | ACO1 |
| Synonyms | ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1 |
| ACCN | NM_002197 |
| Insert Size | 2000 bp |
| Sequence Data | >SC209391 3’UTR clone of NM_002197 The sequence shown below is from the reference sequence of NM_002197. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC AACTACATGATCCGCAAGATGGCCAAGTAGGAGACGTGCACTTGGTGCTGCGCCCAGGGAGGAAGCCGC ACCACCAGCCAGCGCAGGCCCTGGTGGAGAGGCCTCCCTGGCTGCCTCTGGGAGGGGTGCTGCCTTGTA GATGGAGCAAGTGAGCACTGAGGGTCTGGTGCCAATCCTGTAGGCACAAAACCAGAAGTTTCTACATTC TCTATTTTTGTTAATCATCTTCTCTTTTTCCAGAATTTGGAAGCTAGAATGGTGGGAATGTCAGTAGTG CCAGAAAGAGAGAACCAAGCTTGTCTTTAAAGTTACTGATCACAGGACGTTGCTTTTTCACTGTTTCCT ATTAATCTTCAGCTGAACACAAGCAAACCTTCTCAGGAGGTGTCTCCTACCCTCTTATTGTTCCTCTTA CGCTCTGCTCAATGAAACCTTCCTCTTGAGGGTCATTTTCCTTTCTGTATTAATTATACCAGTGTTAAG TGACATAGATAAGAACTTTGCACACTTCAAATCAGAGCAGTGATTCTCTCTTCTCTCCCCTTTTCCTTC AGAGTGAATCATCCAGACTCCTCATGGATAGGTCGGGTGTTAAAGTTGTTTTGATTATGTACCTTTTGA TAGATCCACATAAAAAGAAATGTGAAGTTTTCTTTTACTATCTTTTCATTTATCAAGCAGAGACCTTTG TTGGGAGGCGGTTTGGGAGAACACATTTCTAATTTGAATGAAATGAAATCTATTTTCAGTGAAAACTTG TTGACTTTGAGTTTTGCTGTGTTTGTGGCTAGAGTTTTGGGATATTTAGTACAGAGTGAATCTCACACC ATATCATTGGGAAGCCTGAATAACCTTCATATTCTCCCATTTTTACAACTCTATCAGAACTGTACGGGT ATAACGGAAATGTTTAGGAACCATTATGCTCGCTCTTCCCTGTTTGGGAGCAGACAAGAAGGAAGAGTA GCCTGGTGGCAGTGGCAACGATATAAGTACATAAGGAAAAGCAGATAAACTTCAGGACACTAAAAACCA ATTATCTGCTCATTTTTACCCCTGTAGTCATTTCTGGGCATGCCTTAGTTTGCTTGGGTTGCTATAATG TAATGCCATAGACTGGCTGAAAGAACAGAAATGTATTTTCTCACAGTTATGGAGGCTGGAATTCTGAGA TCAAAGTTGAAGCCACTTCATTTTCTGGTGAGGACACTCTTCCTGGTTTACAGATGGCCACCTTCTTGC TTGTTCTCACATGGCATTTCTTTGGTATGTGCCTGGTGGGGGAGAGAGAAAATAGGCTCTCATGTCTCT TCTTACAGCAACACTAATTGTATTGGATCAGGGTCCCACTCTTATGGAGACCCAGGAAAGCTGGTGGTA TAACAACTTGGTTCAAGTCCAAATGCCTAAGAACCTGGGGAGGGGCATGGGGAATGCTGGTATAAGTCC CAGAGTCTGAATGCCCAGGAACAAGCAGGAGCTGTGATGTCCAAGGACAGGAGGGCAGGATCTTTGTAA TGAATTTTTAATGTCAACCTTAGCACTCAGGGGATATGGCCTGGTCATCAGAACATCTGGAATCTAATC CTCTTTCTACTTCTCACCAGATGAGGGATCAAGACAGGTTATTTAGCATCCCTGCACTTGCGGTCCTGG GGATCTTATTTATATTCATTTAAAAAATAAATTACAAACAAACAGCCAGGCGCAGTGGCTCATGCCTAT AATTCCATCACTTTGGGAGGCCGAGGTGGGTAGATCACCTGAGGTCAGGAGTTCTAGACCAGCCTGGCC AACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAAATTAGCCAGGTGTGGTATTGTGTGCCTGTAA TCCCAGCTACACAGGAGGCTGAGGCAGGAGAATTGCTTAAACCCGGGATGCAGAGGCTGCAGTGAGCCG AGATCGTGCCATTGCACGCCCGCCTGGGCTACAAGAGCAAAGCTCCGTCTCAAAAAAAAAAAAAAATT ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG |
| Restriction Sites | SgfI-MluI |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
| Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
| Reference Data | |
| RefSeq | NM_002197.3 |
| Summary | The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014] |
| Locus ID | 48 |
| MW | 75.4 |
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