Aquaporin 2 Antibody (Phospho-Ser261)
Cat# OAPC00158
Size : 100ul
Marca : Aviva Systems Biology
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Telefono : +1 850 650 7790
Aquaporin 2 Antibody (Phospho-Ser261) (OAPC00158)
| Datasheets/Manuals | Printable datasheet for OAPC00158 |
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| Predicted Species Reactivity | Human, Mouse, Rat, Bovine, Chicken, Canine, Non-human primate |
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| Product Format | Liquid.10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol. Adequate amount of material to conduct 10 mini Western blots |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB, DB, IHC |
| Reconstitution and Storage | For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freezing/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of rat aquaporin 2. |
| Purification | The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns. |
| Concentration | 0.52 mg/ml |
| Specificity | Specific for ~29k AQP2 protein phosphorylated at Ser261 in Western blots of rat kidney lysates. |
| Application Info | WB: 1:1000 DB: 1:1000 IHC: 1:100 Optimal working dilutions should be determined by the end user. |
| Reference | Hoffert JD, Nielsen J, Yu MJ, Pisitikun T, Schleicher SM, Nielsen S, Knepper MA (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700. Hoffert JD, Fenton RA, Moeller HB, Simons B, Tchapyjnikov D, McDill BW, Yu MJ, Pisitkun T, Chen F, Knepper MA. (2008) Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27 |
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| Gene Symbol | AQP2 |
| Alias Symbols | AQP-2, aquaporin-2 |
| NCBI Gene Id | 25386 |
| Protein Name | Aquaporin-2 |
| Description of Target | Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007). |
| Uniprot ID | P34080 |
| Protein Accession # | NP_037041.2 |
| Molecular Weight | ~29 kDa |
