ATP Colorimetric Assay Kit
Cat# E-BC-K157-S-100
Size : 100Assays
Marca : Elabscience
Detection Principle | Creatine Kinase catalyzes adenosine triphosphate and creatine to produce creatine phosphate, then detected by phosphomolybdic acid colorimetry. |
Synonyms | ATP |
Sample Type | Tissue |
Detection Method | Colorimetric method |
Detection Instrument | Spectrophotometer (636 nm) |
Research Area | Oxidative Stress , Energy Metabolism |
Other Reagents Required | Deionized water, Normal saline (0.9% NaCl) |
Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
Valid Period | 12 months |
Sensitivity | 0.01 mmol/L |
Detection Range | 0.01-1.5 mmol/L |
Precision | inter-assay CV: 9.3% | intra-assay CV: 3.6% |
Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
Sample Type | Dilution Factor |
---|---|
10% Rat muscle tissue homogenate | 2-3 |
10% Rat liver tissue homogenate | 2-3 |
10% Mouse brain tissue homogenate | 2-3 |
10% Rat kidney tissue homogenate | 2-3 |
10% Rat lung tissue homogenate | 2-3 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
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