Sodium azide inhibits Gram negative and Gram positive bacteria, other than enterococci, are inhibited by the bile salts.||Bile Esculin Agar is prepared according to the formula specified by Swan, and later evaluated by Facklam and Moody. It was concluded that the bile esculin test provided a reliable means of identifying enterococci and differentiation from non-enterococci. Bile Esculin Azide Agar is a more selective medium as described by lsenberg. Enterococci are bile tolerant and hydrolyze esculin which imparts a dark brown color to the medium. Sodium azide inhibits Gram negative and Gram positive bacteria other than enterococci are inhibited by the bile salts.||Appearance: |Beige, homogeneous. free flowing powder||Solubility: |Grayish yellow, opalescent/trace to light haze||pH (5.7%): |7.1 ± 0.2 ||Growth Performance:|Note: Growth results obtained from type cultures after incubation at 35°C and observed after 18-24 hours.||Escherichia coli (ATCC 25922): Inhibited|Entertococcus fecalis (ATCC 29212): Good Growth, Blackening of the medium||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.