Catenin, beta (FITC)

Adherens junctions (AJ; also referred to as zonula adherens) are required for both the establishment and maintenance of epithelial layers (1). In addition, these junctions have been identified in several other cell types including cardiac myocytes and fibroblasts (1). AJ subserve several important functions including: mediating intercellular adhesion, sensing the presence of neighboring cells, and anchoring the actin cytoskeleton (1,10). AJ are multiprotein complexes that are assembled around cell adhesion molecules called cadherins. Cadherins are a multifunctional family of Ca2+-dependent, transmembrane glycoproteins which promote cell-cell adhesion (6). The cadherin extracellular domain mediates homophilic interactions between like cadherin molecules on neighboring cells, while the intracellular domain interacts with several cytoplasmic proteins which include: a-catenin, b-catenin, g-catenin (plakoglobin), and the tyrosine kinase substrate p120cas (2,4,7,9,13,16). Cadherin-catenin interactions are required for complete cadherin activity and regulate the interaction between cadherins and the actin-based cytoskeleton (2,4,6,9,13,17). In fact, deletion of the cadherin cytoplasmic domain produces an adhesion-defective molecule that is unable to interact with the cytoskeleton (6,8). ||Applications: |Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Immunofluorescence: 5–10ug/ml |Western Blot: 1ug/ml|Immunoprecipitation: 2–5ug |Immunohistochemistry (Formalin-fixed and paraffin-embedded tissue): 2–10ug/ml. Requires heat induced epitope retrieval. |Optimal dilutions to be determined by the researcher.||Positive Control:|HeLa, A431 and WI-38 human cell lines||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.