CD8 (HRP)

Equine homologue of the human CD8 cell surface antigen which is expressed by a subset of T lymphocytes.||A study undertaken using CVS8 to identify CD8 on several wild african equid species indicates that the clone recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra) (Ibrahim 2007).||Applications: |Suitable for use in Flow Cytometry (Not Tested), Immunoprecipitation, Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Flow Cytometry (Not Tested): 1:25-1:200|Optimal dilutions to be determined by the researcher.||Hybridoma: |X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.