CD86 (B70, B7-2, CD28LG2, CTLA-4 Counter-receptor B7.2, LAB72 T-lymphocyte Activation Antigen CD86 Precursor) (PE)

Cat# C2438-01H-100T

Size : 100Tests

Marca : US Biological

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C2438-01H CD86 (B70, B7-2, CD28LG2, CTLA-4 Counter-receptor B7.2, LAB72 T-lymphocyte Activation Antigen CD86 Precursor) (PE)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P42081
Isotype
IgG1
Grade
Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: 20ul labels 10e6 cells in 100ul|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 7H172 (BU63)|Host: mouse|Source: human|Concentration: Not Determined |Form: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with R-Phycoerythrin (PE). |Purity: Purified|Immunogen: B-lymphoblastoid cell line ARH 77|Specificity: Recognizes human CD86.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
B-lymphoblastoid cell line ARH 77
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with R-Phycoerythrin (PE).
Purity
Purified
Specificity
Recognizes human CD86.
References
1. Zhan H, Towler HM, Calder VL: The immunomodulatory role of human conjunctival epithelial cells. Invest Ophthalmol Vis Sci. 2003 Sep;44(9):3906-10.|2. Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses. BMC Immunol. 2011 Jan 5;12:2.|3. Caux C, Vanbervliet B, Massacrier C, Azuma M, Okumura K, Lanier LL, Banchereau J: B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med. 1994 Nov 1;180(5):1841-7.|4. Giguère JF, Bounou S, Paquette JS, Madrenas J, Tremblay MJ: Insertion of host-derived costimulatory molecules CD80 (B7.1) and CD86 (B7.2) into human immunodeficiency virus type 1 affects the virus life cycle. J Virol. 2004 Jun;78(12):6222-32.