Cellulase (Onozuka R-10), Trichoderma viride

Cellulase (Onozuka R-10) is a multi-component enzyme system derived from Trichoderma viride and contains cellulase, alpha-amylase, hemicellulase, pectinase and protease activities. It is often used in the isolation of plant protoplasts in combination with other enzymes.||Source: |Trichoderma viride||Synonyms: |Onozuka R-10||CAS No: |9012-54-8||EC Number:|3.2.1.4||Unit Definition of Activity: |One unit of Cellulase will liberate 1.0 micromole of glucose form carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).||Major Enzyme Activity:|Cellulase: 1u/mg||Extraneous Enzyme Activities:|Pectinase: 0.4u/mg|Hemicellulase: 1.0u/mg|alpha-Amylase: 0.6u/mg|Protease: 0.01u/mg||Optimum pH range: |4-5||Appearance:|Light yellow powder||Solubility:|Soluble in water (10mg/ml)||Loss on Drying:|≤10%||Arsenic:|≤3ug||Heavy Metals:|≤40ug||Activity:|≥10,000U/g||Total Viable Count:|≤10,000cfu/g||Bacteria:|≤10,000cfu/g||Fungi:|<100cfu/g||Coliform:|Negative||Stability and Storage:|Lyophilized powder may be stored at 4°C. Stable for 6 months at 4°C.