CF®488A Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL

Cat# 20014

Size : 500uL

Marca : Biotium

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Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat)

Highly cross-adsorbed donkey anti-mouse IgG (H L) secondary antibody labeled with our superior CF® Dyes and other labels.

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Secondary Antibody Product Listings

Tech Tips

Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

Product Description

This is a highly cross-adsorbed donkey anti-mouse IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes and other labels. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, rat and sheep serum proteins.

  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 17 bright and photostable CF® Dyes
  • Biotin, AP, and HRP conjugates also available
  • Suitable for western, immunofluorescence, and immunohistology

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat)

Conjugation Ex/Em Size Catalog No. Dye Features
CF®350 347/448 nm 50 uL (100 ug) 20350-1 CF®350 Features
0.5 mL (1 mg) 20350
1 mg (lyophilized) 20350-1mg
CF®430 426/498 nm 50 uL (100 ug) 20461-50uL CF®430 Features
0.5 mL (1 mg) 20461-500uL
1 mg (lyophilized) 20461-1mg
CF®488A 490/515 nm 50 uL (100 ug) 20014-1 CF®488A Features
0.5 mL (1 mg) 20014
1 mg (lyophilized) 20014-1mg
CF®514 516/548 nm 50 uL (100 ug) 20483-50uL CF®514 Features
0.5 mL (1 mg) 20483-500uL
1 mg (lyophilized) 20483-1mg
CF®543 541/560 nm 50 uL (100 ug) 20305-1 CF®543 Features
0.5 mL (1 mg) 20305
1 mg (lyophilized) 20305-1mg
CF®555 555/565 nm 50 uL (100 ug) 20037-1 CF®555 Features
0.5 mL (1 mg) 20037
1 mg (lyophilized) 20037-1mg
CF®568 562/583 nm 50 uL (100 ug) 20105-1 CF®568 Features
0.5 mL (1 mg) 20105
1 mg (lyophilized) 20105-1mg
CF®583R 585/609 nm 50 uL (100 ug) 20894-50uL CF®583R Features
0.5 mL (1 mg) 20894-500uL
CF®594 593/614 nm 50 uL (100 ug) 20115-1 CF®594 Features
0.5 mL (1 mg) 20115
1 mg (lyophilized) 20115-1mg
CF®633 630/650 nm 50 uL (100 ug) 20124-1 CF®633 Features
0.5 mL (1 mg) 20124
1 mg (lyophilized) 20124-1mg
CF®640R 642/662 nm 50 uL (100 ug) 20177-1 CF®640R Features
0.5 mL (1 mg) 20177
1 mg (lyophilized) 20177-1mg
CF®647 650/665 nm 50 uL (100 ug) 20046-1 CF®647 Features
0.5 mL (1 mg) 20046
1 mg (lyophilized) 20046-1mg
CF®660R 663/682 nm 50 uL (100 ug) 20388-50uL CF®660R Features
0.5 mL (1 mg) 20388-500uL
1 mg (lyophilized) 20388-1mg
CF®680R 680/701 nm 50 uL (100 ug) 20194-1 CF®680R Features
0.25 mL (500 ug) 20194
CF®740 742/767 nm 50 uL (100 ug) 20991-50uL CF®740 Features
0.25 mL (500 ug) 20991-250uL
CF®790 784/806 nm 50 uL (100 ug) 20363-50uL CF®790 Features
CF®800 797/816 nm 50 uL (100 ug) 20835-50uL CF®800 Features
Biotin N/A 50 uL (100 ug) 20190-1
0.5 mL (1 mg) 20190
1 mg (lyophilized) 20190-1mg
HRP N/A 100 uL (100 ug) 20404-100uL
1 mL (1 mg) 20404-1mL
1 mg (lyophilized) 20404-1mg
AP N/A 100 uL (100 ug) 20466-100uL
1 mL (1 mg) 20466-1mL

View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of curated CF® Dye references.

 

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Cat#
Descrizione
Cond.
Priced
583-FOX3
 100uL