R1013-1mg | EZ Cap™ Firefly Luciferase mRNA (5-moUTP) Clinisciences

EZ Cap™ Firefly Luciferase mRNA (5-moUTP) will express luciferase protein once entering cells, which is initially extracted from firefly Photinus pyralis. This enzyme catalyzes ATP-dependent D-luciferin oxidation and lead to yield chemiluminescence at about 560 nm wavelength. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc.

EZ Cap™ Firefly Luciferase mRNA (5-moUTP) is provided at a concentration of ~1 mg/ml with Cap1 structure. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of 5-moUTP and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.

1. Min Tang, Ayane Sagawa, et al. "Efficient mRNA Delivery with mRNA Lipoplexes Prepared Using a Modified Ethanol Injection Method." Pharmaceutics. 2023 Apr 4;15(4):1141. PMID: 37111627
2. Pengxuan Zhao, Yu Tian, et al. "Biomimetic calcium carbonate nanoparticles delivered IL-12 mRNA for targeted glioblastoma sono-immunotherapy by ultrasound-induced necroptosis." J Control Release. PMID: 36496387
3. Yu Zhang, Xiaodong Xi, et al. "Chemically modified in-vitro-transcribed mRNA encoding thrombopoietin stimulates thrombopoiesis in mice." Mol Ther Nucleic Acids. 2022 Aug 13;29:657-671. PMID: 36090760

Related Biological Data

EZ Cap™ Firefly Luciferase mRNA (5-moUTP)

Related Biological Data

mRNA Length	1921 nucleotides
Concentration	1 mg/mL
Buffer	1 mM Sodium Citrate, pH 6.4	Storage	-40°C or below
General tips	Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent.
Shipping Condition	Evaluation sample solution: ship with dry ice.

EZ Cap™ Firefly Luciferase mRNA (5-moUTP)

Cat# R1013-1mg

Contatta il distributore locale :

Marca : ApexBio Technology

Contatta il distributore locale :

Background

Product Citation

Quality Control

Related Biological Data

Related Biological Data

Related Biological Data

Description

Si può anche essere interessati nei seguenti prodotti: