Fibronectin (Cold insoluble globulin, CIG, FINC, FN1, FNZ, GFND, GFND2, LETS, Migration Stimulating Factor, MSF, Ugl-Y3)

Fibronectin is a dimeric glycoprotein of 440kD, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.||Applications: |Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.||Recommended Dilution: |Flow Cytometry: 0.5-1ug/million cells |Immunofluorescence: 0.5-1ug/ml |Western Blot: 0.5-1ug/ml|Immunoprecipitation: 0.5-1µg/500ug protein lysate|Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT|(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)|Optimal dilution for a specific application should be determined.||Positive Control:|SW156 cells or Kidney||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.