GelRed® is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.

• Safer than EtBr: non-mutagenic and non-hazardous for disposal
• Much more sensitive than EtBr and SYBR® Safe
• Stable at room temperature and microwavable
• Simple precast or post-electrophoresis gel staining, no destaining
• Replaces EtBr with no change to equipment or optical settings
• Compatible with downstream gel purification, restriction digest, sequencing, and cloning

GelRed®: A Superior Alternative to EtBr

GelRed® is much more sensitive than EtBr, and can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining without destaining. GelRed® can also be used to stain polyacrylamide gels via post gel staining. GelRed® is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning. GelRed® and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed® without changing your existing imaging system. For detailed protocols for use, please download the GelRed® Product Information Sheet. Also see our GelRed® and GelGreen® Frequently Asked Questions (FAQs).

Non-Mutagenic & Safer for the Environment

A series of safety tests have confirmed that GelRed® is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, working strength GelRed® can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For more information, download the GelRed®/GelGreen® Safety Report or our  Gel Stains Comparison White Paper.

Need a Safe & Sensitive Gel Imager?

Biotium’s Gel-Bright™ Laser-Diode Gel Illuminator offers exceptional performance for imaging GelRed® or GelGreen® stained agarose gels without hazardous UV light. Each purchase of the Gel-Bright™ also comes with a free sample of GelRed® Agarose LE (Cat. No. 41029-5G) and GelGreen® Agarose LE (Cat. No. 41030-5G).

Choose the Right Stain for Your Application

For agarose gels, we recommend using Original GelRed® Nucleic Acid Gel Stain or GelGreen® Nucleic Acid Gel Stain. GelRed® 3X in water is ready-to-use for post-electrophoresis gel staining, and is supplied in a 4L Cubitainer®. Higher concentrations of Original GelRed® are available as 10,000X in water or DMSO. GelRed® in water is a newer, safer formulation and our recommended format. We continue to offer GelRed® in DMSO for established users who do not wish to alter their protocols. We also offer GelRed® Agarose and GelRed® Prestain Plus 6X Loading Dye.

Also see GelGreen® Nucleic Acid Gel Stain, a safer replacement for SYBR® gel stains, which is compatible with visible light excitation. Also learn about our Go-Go™ Fast DNA Gel Running Buffer for running gels 3X faster than with TAE or TBE buffer.

For more information, view our DNA Gel Stains Technology Page.

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