AP9839b-ev | GLG1 Antibody (C-term) Clinisciences

GLG1 Antibody (C-term) (Cat. #AP9839b) western blot analysis in HepG2,T47D cell line lysates (35ug/lane).This demonstrates the GLG1 antibody detected the GLG1 protein (arrow).
Western blot analysis of GLG1 Antibody (C-term) (Cat. #AP9839b) in mouse cerebellum tissue lysates (35ug/lane). GLG1 (arrow) was detected using the purified Pab.
All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: mouse cerebellum lysate Lane 3: MCF-7 whole cell lysate Lane 4: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution Lane 1: MCF-7 lysate Lane 2: HepG2 whole cell lysate Lane 3: T47D whole cell lysate Lane 4: mouse cerebellum lysate Lane 5: human placenta lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-GLG1 Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 136 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
GLG1 Antibody (C-term) (Cat. #AP9839b) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GLG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, IHC-P, E
Primary Accession	Q92896
Other Accession	Q62638, Q61543, Q9Z1E9, Q02391
Reactivity	Human, Mouse
Predicted	Chicken, Hamster, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	134552 Da
Antigen Region	1152-1179 aa

Additional Information
Gene ID	2734
Other Names	Golgi apparatus protein 1, CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand 1, ESL-1, Golgi sialoglycoprotein MG-160, GLG1, CFR1, ESL1, MG160
Target/Specificity	This GLG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1179 amino acids from the C-terminal region of human GLG1.
Dilution	WB~~1:2000 IHC-P~~1:50~100
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GLG1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GLG1
Synonyms	CFR1, ESL1, MG160
Function	Binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils).
Cellular Location	Golgi apparatus membrane; Single-pass type I membrane protein. Golgi outpost {ECO:0000250\|UniProtKB:Q62638}. Cytoplasm, cytoskeleton, microtubule organizing center {ECO:0000250\|UniProtKB:Q62638}. Note=Golgi medial cisternae. Localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, which shapes dendrite morphology by functioning as sites of acentrosomal microtubule nucleation. {ECO:0000250\|UniProtKB:Q62638}
Tissue Location	Widely expressed. Highest levels in pancreas, skeletal muscle, placenta, heart, testis and ovary. Also found in the kidney, liver, lung and brain.

GLG1 Antibody (C-term)

Cat# AP9839b-ev

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Marca : Abcepta

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GLG1 Antibody (C-term)

Cat# AP9839b-ev

Contatta il distributore locale :

Marca : Abcepta

Contatta il distributore locale :

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