Histone H3 K36me1 antibody
Cat# orb420413-50ug
Size : 50ug
Marca : Biorbyt
Histone H3 K36me1 antibody
Catalog Number: orb420413
Catalog Number | orb420413 |
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Category | Antibodies |
Description | Histone H3 K36me1 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ChIP, DOT, IF, IHC, Multiplex Assay, WB |
Reactivity | C. elegans, Human |
Isotype | IgG |
Immunogen | Histone H3 [Monomethyl Lys36] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 36 of human Histone H3.2. |
Concentration | 0.45 mg/ml |
Dilution range | ChIP: 2-5µg/million cells, IHC: 1:500, IF: 1:500, WB: 1:1000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | Anti-Histone H3 [Monomethyl Lys36] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q71DI3 |
NCBI | NP_001005464 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Histone H3 monomethyl Lys36 antibody, |
Note | For research use only |
Application notes | Anti-Histone H3 [Monomethyl Lys36] antibody is tested in Western Blot, Dot Blot, and Immunofluorescence. This antibody is useful for Chromatin Immunoprecipitation and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals. |
Expiration Date | 12 months from date of receipt. |
Dot Blot of Rabbit Histone H3 [Monomethyl Lys36] Antibody. Lane 1: K36. Lane 2: K36Me1. Lane 3: K36Me2. Lane 4: K36Me3. Lane 5: K36ac. Load: 0.3, 0.6, 1, 3, 6, and 10 picomoles of peptide. Primary antibody: Histone H3 [Monomethyl Lys36] antibody at 0.5 µg/ml for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
Immunofluorescence of Rabbit Anti-Histone H3 [Monomethyl Lys36] Antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: Not required. Primary antibody: Histone H3 [Monomethyl Lys36] antibody at a 1:100 dilution for 1 h at RT. Secondary antibody: Dylight 488 secondary antibody at 1:10000 for 45 min at RT. Localization: Histone H3 [Monomethyl Lys36] is nuclear and chromosomal. Staining: Histone H3 [Monomethyl Lys36] is expressed in green, nuclei and alpha-tubulin are counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys36] Antibody. Lane 1: C. elegans embryo lysate. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys36] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.
Western Blot of Rabbit Anti-Histone H3 [Monomethyl Lys36] Antibody. Lane 1: HeLa histone preps. Load: 30 µg per lane. Primary antibody: Histone H3 [Monomethyl Lys36] at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15 kDa. Other band(s): None.