Human alpha 1 Fetoprotein (AFP) ELISA Kit 1 x 96

CAT#: EA200031

Human alpha 1 Fetoprotein (AFP) ELISA Kit 1 x 96



Product Manual for 'EA200031'

Product Images

Specifications

Product Data
Format 8x12 divisible strips
Assay Type Sandwich
Assay Length 3.5 hours incubations; 0.5 hour washing and analyzing samples
Signal Colorimetric
Sample Type Human serum, plasma and other biological fluids.
Sample Volume 100µl
Specificity This kit is used for quantitative detection of AFP
Sensitivity 0.13ng/ml
Reactivities Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • AFP Antibody Coated 96-well Plate in foil pouch with desiccant|1 plate
  • Recombinant Human AFP Standard (1.25µg/ml)|0.1 mL
  • 100x Biotin conjugated AFP Detection Antibody|0.12 mL
  • 100x SA-HRP Conjugate0.12 mL
  • Assay Buffer|30 mL
  • Standard&Sample Diluent|30 mL
  • Wash Buffer Concentrate 20X|60 mL
  • TMB Substrate|12 mL
  • Stop Solution|12 mL
  • Plate Sealer|3 pieces
Background Alpha-fetoprotein (AFP) is a protein encoded by the AFP gene. AFP is a glycoprotein of 591 amino acids, found in monomeric, dimeric and trimeric forms. AFP is a major plasma protein produced by the yolk sac and the fetal liver during fetal development. AFP binds to copper, nickel, fatty acids and bilirubin. Serum AFP level is elevated in people with developmental birth defects such as omphalocele, gastroschisis and neural tube defects, hepatocellular carcinoma, metastatic disease affecting the liver, yolk sac tumor, nonseminomatous germ cell tumors, and ataxia telangiectasia. AFP has been used as a biomarker to these diseases.
Gene Symbol AFP
Gene ID 174
Standard Curve
Data image of Human alpha 1 Fetoprotein (AFP) ELISA Kit.