- Product Name
- Human anti-SARS-CoV2(N) IgG ELISA Kit
- Alias
- SARS-CoV-2(N) IgG ELISA Kit
- Catalogue No.
- EH4397
- Size
- 48T/96T
- Species
- Human
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Indirect ELISA
- Detection Wavelength
- OD450
- Reaction Duration
- 1.5 hours
- Range
- 3.906-250ng/ml
- Sensitivity
- 2.344ng/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with anti-SARS-CoV2 (N) IgG , no obvious cross reaction with other analogues.
- Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
-
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(ng/ml) | OD-1 | OD-2 | Average |
0 | 0.05 | 0.053 | 0.051 |
3.906 | 0.203 | 0.213 | 0.207 |
7.813 | 0.336 | 0.353 | 0.343 |
15.625 | 0.554 | 0.583 | 0.566 |
31.25 | 0.9 | 0.945 | 0.918 |
62.5 | 1.246 | 1.309 | 1.271 |
125 | 1.719 | 1.807 | 1.754 |
250 | 2.05 | 2.154 | 2.092 |
- Recovery
-
Add a certain amount of anti-SARS-CoV2 (N) IgG into the sample. Calculate the recovery by comparing the measured value with the expected amount of anti-SARS-CoV2 (N) IgG in the sample.
Sample Type | Recovery Range(%) | Average(%) |
serum(n=10) | 88-104 | 96 |
EDTA plasma(n=10) | 87-103 | 93 |
Heparin plasma(n=10) | 87-105 | 95 |
- Linearity
-
Dilute the sample with a certain amount of anti-SARS-CoV2 (N) IgG at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type | 1:2 | 1:4 | 1:8 |
serum(n=10) | 85-97% | 86-98% | 86-104% |
EDTA plasma(n=10) | 84-101% | 85-98% | 91-98% |
Heparin plasma(n=10) | 88-100% | 83-97% | 80-97% |
- Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item | Intra-assay Precision | Inter-assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/ml) | 7.3 | 29.22 | 123.12 | 7.67 | 29.93 | 124.1 |
Standard deviation | 0.32 | 1.3 | 5.76 | 0.34 | 1.37 | 5.78 |
CV(%) | 4.43 | 4.46 | 4.68 | 4.37 | 4.59 | 4.66 |
- Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) | 37°C for 1 month | 2-8°C for 6 months |
Average(%) | 80 | 95-100 |