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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat # MAB12811) at the following concentrations: 0.1 ug/mL, 0.02 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both kappa and lambda light chains of human immunoglobulins; no cross reactivity with mouse, rat, or goat immunoglobulin light chain. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against human Ig light chain.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against human IgG.

    Sequence

    N/A

    Reactivity

    Human

    Specificity

    This antibody reacts to both kappa and lambda light chains of human immunoglobulins. No cross reactivity with mouse, rat, or goat immunoglobulin light chain.

    Form

    Liquid

    Conjugation

    Biotin

    Purification

    Protein A affinity purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.02-0.2 ug/mL)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry

    Immunocytochemistry

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Human Ig light chain monoclonal antibody, clone RM129 (Biotin) (Cat # MAB12811) at the following concentrations: 0.1 ug/mL, 0.02 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to both kappa and lambda light chains of human immunoglobulins; no cross reactivity with mouse, rat, or goat immunoglobulin light chain. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info — IGKC

    Entrez GeneID

    3514

    Gene Name

    IGKC

    Gene Alias

    HCAK1, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

    Gene Description

    immunoglobulin kappa constant

    Omim ID

    147200

    Gene Ontology

    Hyperlink

    Other Designations

    -

  • Gene Info — IGLC1

    Entrez GeneID

    3537

    Gene Name

    IGLC1

    Gene Alias

    IGLC

    Gene Description

    immunoglobulin lambda constant 1 (Mcg marker)

    Omim ID

    147220

    Gene Ontology

    Hyperlink

    Other Designations

    -

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  • Diseases