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> HUMAN IgG F(ab')2

HUMAN IgG F(ab')2

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Cat# ASR2302

Size : 2mg

Marca : Abcepta

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  • - HUMAN IgG F(ab')2 ASR2302
    SDS-Page of Human IgG F(ab’)2 Fragment. Lane 1: Human F(ab’)2 – Non-Reduced. Lane 2: Human F(ab’)2 – Reduced. Load: 1.0 µg per lane. Predicted/observed size: 25 kDa – Reduced, 120 kDa – Non-Reduced for F(ab’)2 fragment. Other band(s): None.
Product Information
Description HUMAN IgG F(ab')2 fragment
Conjugate Unconjugated
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Application Note ELISA 1:5000-1:50,000
Western Blot 1:1000
Physical State Lyophilized
Host Isotype IgG F(ab')2
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin Human
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity Human IgG F(ab')2 was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum, anti-Human IgG and anti-Human IgG F(ab’)2.   No reaction was observed against anti-Human IgG F(c) or anti-Pepsin.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.