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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Human IgM monoclonal antibody, clone RM121 (Cat # MAB12812) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgM; no cross reactivity human IgG, IgE, IgD, or IgA. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against human IgM.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against human IgM.

    Sequence

    N/A

    Reactivity

    Human

    Specificity

    This antibody reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE.

    Form

    Liquid

    Purification

    Protein A affinity purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.05-0.2 ug/mL for detection; 0.05-0.2 ug/well for capture)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry

    Immunocytochemistry

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Human IgM monoclonal antibody, clone RM121 (Cat # MAB12812) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgM; no cross reactivity human IgG, IgE, IgD, or IgA. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info — IGHM

    Entrez GeneID

    3507

    Protein Accession#

    P01871

    Gene Name

    IGHM

    Gene Alias

    DKFZp686I15196, DKFZp686I15212, FLJ00385, MGC104996, MGC52291, MU, VH

    Gene Description

    immunoglobulin heavy constant mu

    Omim ID

    147020 601495

    Gene Ontology

    Hyperlink

    Other Designations

    -

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  • Diseases