Kasumi-1
Marca : CLS Cell Lines Service
Kasumi-1 Cells
General information
Description | The Kasumi-1 cell line was derived from the peripheral blood of a 7-year-old Japanese boy with AML (FAB M2) in relapse after bone marrow transplantation. Kasumi-1 cells have the characteristics of myeloid and macrophage lineages. they differentiate into macrophage-like cells when cultured with TPA. |
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Organism | Human |
Tissue | Blood |
Disease | Acute myeloblastic leukemia |
Synonyms | KASUMI-1, Kasumi 1, KASUMI1, Kasumi1 |
Characteristics
Age | 7 years |
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Gender | Male |
Ethnicity | Japanese |
Morphology | Round cells showing marked variations in both size and nuclear cytoplasmic ratio. |
Cell type | Myeloblast (AML-acute myeloid leukemia) |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | Kasumi-1 (Cytion catalog number 300226) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | CD4+ (37.1%, coexpressed with CD34 and CD33), CD13+(OKM13), CD15+(LeuM1), CD33+, CD34+(MY10), CD38+(OKT10, 50.1%), CD71+(Nu-TERf), HLA-DR+(OKDR). |
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Karyotype | t(8,21) chromosome translocation |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 40 to 45 hours |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Split ratio | A ratio of about 1:2 to 1:3 every 3 to 4 days is recommended |
Seeding density | 1 x 10^5 cells/ml |
Fluid renewal | Add fresh medium (20 to 30% by volume) every 2 to 3 days |
Freezing recovery | About one week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 10,12 D13S317: 11,13 D16S539: 9,12 D5S818: 9,11 D7S820: 8,11 TH01: 6,9 TPOX: 8,9 vWA: 14 D3S1358: 15,17 D21S11: 30,31 D18S51: 15,16 Penta E: 11 Penta D: 12 D8S1179: 13,14 FGA: 22,24 |
HLA alleles | A*: 26:01:01, 26:02:01 B*: 40:06:01, 48:01:01 C*: 03:03:01, 08:01:01 DRB1*: 09:01:02, 14:54:01 DQA1*: 01:04:01, 03:02:01 DQB1*: 03:03:02, 05:03:01 DPB1*: 02:01:02, 02:01:02 E: 01:03:01 |