Mannose ELISA Kit

Cat# OKEH02595

Size : 96Wells

Marca : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02595
Product Info
Predicted Species ReactivityAll Species
ApplicationELISA-Comp
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Mannose ELISA Kit (OKEH02595) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mannose antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Mannose and incubated. The Mannose found in the sample or standards competes with the biotinylated Mannose for limited binding sites on the immobilized anti-Mannose antibody. Excess unbound biotinylated Mannose and sample or standard Mannose is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Mannose captured in the well and inversely proportional to the amount of Mannose which was contained in the sample or standard.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.2% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Mannose Microplate96 Wells (12 x 8 Well strips)
Mannose Lyophilized Standard2 x 100 ng
Sample Diluent1 x 20 mL
100X Mannose-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: MN
Target Name: Mannose
::Pubchem: 18950
::Chemical formula: C6H12O6
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.37 ng/mL
SpecificityNatural and recombinant General Mannose
Publications

Elevated Serum Mannose Levels as a Marker of Polycystic Ovary Syndrome. Front Endocrinol (Lausanne). 10, 711 (2019). 31681178

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsMN, Mannose, MN
Protein NameMannose
Description of TargetMannose is a sugar monomer of the aldohexose series of carbohydrates. Mannose is a C-2 epimer of glucose. Mannose is important in human metabolism, especially in the glycosylation of certain proteins. Several congenital disorders of glycosylation are associated with mutations in enzymes involved in mannose metabolism.

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