Mouse Anti-Human CD3-PE
Cat# 9515-09S
Size : 25tests
Marca : Southern Biotech
Contatta il distributore locale :
Telefono : +1 850 650 7790
| Clone | UCHT1 |
|---|---|
| Isotype | Mouse (BALB/c) IgG1κ |
| Isotype Control | Mouse IgG1-PE (15H6) |
| Specificity | Human CD3 |
| Alternative Names | CD3ε, T3E, TCRE, Leu-4, T3 |
| Description | CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. The monoclonal antibody UCHT1 reacts with the 20 kDa ε chain of the CD3/TCR complex. |
| Immunogen | Human peripheral blood lymphocytes from a patient with Sézary's syndrome and infant thymocytes |
| Conjugate | PE (R-phycoerythrin) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide and a stabilizer |
| Clonality | Monoclonal |
| Concentration | Lot specific |
| Volume | 0.25 mL or 1.0 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light; Do not freeze |
| Applications | Flow Cytometry – Quality tested 1,6-11 Immunohistochemistry-Frozen Sections – Reported in literature 4,5 Immunohistochemistry-Paraffin Sections – Reported in literature 5 Immunocytochemistry – Reported in literature 1 Immunoprecipitation – Reported in literature 2 Western Blot – Reported in literature 3 Activation – Reported in literature 1,2 Blocking – Reported in literature 2 |
| RRID Number | AB_2796831 |
| Gene ID | 916 (Human) |
| Gene ID Symbol | CD3E (Human) |
| Gene ID Aliases | T3E; TCRE; IMD18 |
| UniProt ID | P07766 (Human |
| UniProt Name | CD3E_HUMAN (Human) |
Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup
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- 1. Beverley PC, Callard RE. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981;11:329-34. (Immunogen, ICC, Activ, FC)
- 2. Burns GF, Boyd AW, Beverley PC. Two monoclonal anti-human T lymphocyte antibodies have similar biologic effects and recognize the same cell surface antigen. J Immunol. 1982;129:1451-7. (IP, Activ, Block)
- 3. van Dongen JJ, Krissansen GW, Wolvers-Tettero IL, Comans-Bitter WM, Adriaansen HJ, Hooijkaas H, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988;71:603-12. (WB)
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- 8. Farag MM, Weigand K, Encke J, Momburg F. Activation of natural killer cells by hepatitis C virus particles in vitro. Clin Exp Immunol. 2011;165:352-62. (FC)
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