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> Mouse IgG1 antibody (Peroxidase)

Mouse IgG1 antibody (Peroxidase)

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Cat# orb347380-1mg

Size : 1mg

Marca : Biorbyt

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    Mouse IgG1 antibody (Peroxidase)

    Catalog Number: orb347380

    Catalog Numberorb347380
    CategoryAntibodies
    DescriptionMouse IgG1 antibody (Peroxidase)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityMouse
    IsotypeIgG
    ImmunogenMouse IgG1 heavy chain
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000
    Form/AppearanceLyophilized
    PurityAnti-Mouse IgG1 HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
    ConjugationHRP
    StorageStore vial antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    Alternative namesGoat anti-mouse IgG1 antibody peroxidase conjugati
    NoteFor research use only
    Application notesAnti-Mouse IgG1 Peroxidase Antibody has been tested by ELISA and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
    Expiration Date12 months from date of receipt.
    Mouse IgG1 antibody (Peroxidase)

    ELISA Results of Goat Anti-Mouse IgG1 mx3 Antibody Peroxidase Conjugated tested against purified Mouse IgG1 mx3. Each well was coated in duplicate with 1.0 µg of Mouse IgG1 (p/n orb343776). The working dilution of Mouse IgG1 mx3 HRP is 1:100000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n orb348651).