NADP+/NADPH Assay Kit is an assay kit designed for the detection and quantification of NADP+/NADPH concentrations in tissue homogenates and cell lysates using a microplate reader.

Detecting total NADP+ and NADPH: Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfers electrons to WST-8 to produce a yellow reaction product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP+ and NADPH can be quantified by measuring the OD value at 450 nm.

Detection of NADPH: After treating sample, the sample is heated to decompose any NADP+ in the sample. The remaining NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measuring the OD value at 450 nm.

Detecting NADP+ and NADP+/NADPH: The content of NADP+ and the ratio of NADP+/NADPH in the sample can be obtained according to the total content of NADP+ and NADPH obtained in the first two steps as well as the separate content of NADPH.

Target	NADP/NADPH
Reactivity	General (All species)
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity	The validity for this kit is 12 months.
Test Range	0.02 µmol/L - 5 µmol/L
Sensitivity	0.02 µmol/L
Detection Method	Colorimetric
Assay Data	Quantitative
Sample Type	Tissue homogenates and cell lysates.
CAS Number	53-59-8
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on NADP/NADPH